Trifluoroacetic acid methylene chloride → HCID 178513159, compound

Participants

Reaction Steps

The crude ester 82 was treated with TFA/DCM (0.75 mL/1 mL). After one and half hours, LC-MS analysis indicated completion of the reaction. The solvent was removed and the residue was purified by preparative HPLC (Vydac reverse phase C-18 column, 22×250 mm ID). Mobil phase: A=0.1% TFA in water, B=0.1% TFA in acetonitrile. The flow rate was 12 mL/min. The gradient time was 5% B to 50% B over 45 min. The desired product, 83, was obtained as a white solid (5.5 mg). Anal. C19H24N6O4, Mol. Wt.: 290.28. Found: ESI-MS: 291.0 (M+H)+.

Provenance & Evidence