Sodium Chloride → HCID 57590164, compound

Participants

Reaction Steps

An emulsion of 1.7 g 41a in an aqueous buffer (potassium phosphate 3 mM, NaCl 0.1 M) was adjusted to pH 7.5. Under stirring at room temperature the hydrolysis was performed with 15 mg lipase OF from Candida rugosa under pH control (pH-static, 1M NaOH). After consumption of 6.5 ml 1M NaOH solution and 7 h the reaction mixture was washed with 50 ml t-butyl methyl ether. The aqueous layer was adjusted to pH 2.0 (conc. HCl) and was extracted with 85 ml ethyl acetate. Drying over sodium sulfate, evaporation and drying overnight on a high vacuum yielded 1.28 g of the title compound 41b. MS: 197.2 (M−H)−; chiral GC: ee 99.4%.

Provenance & Evidence