Huagongshe화공사 화학 정보 인쇄 보고서 · 연구 참고용
Sodium Chloride → 2-Isobutyl-3-ketoglutarate diethyl ester
참여 물질
| 역할 | 이름 | HCID | 투입량 |
|---|---|---|---|
| 반응물 | Sodium Chloride | HCID 5234 | — |
| 반응물 | Potassium Phosphate, Tribasic | HCID 62657 | — |
| 반응물 | (2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanal | HCID 107526 | — |
| 반응물 | 2-Isobutyl-3-ketoketoglutarate diethyl ester | HCID 22022910 | — |
| 용매 | Dimethyl Sulfoxide | HCID 679 | — |
| 용매 | Glycerol | HCID 753 | — |
| 용매 | Ethyl Acetate | HCID 8857 | — |
| 생성물 | 2-Isobutyl-3-ketoglutarate diethyl ester | HCID 9992659 | — |
반응 단계
To 40 mL of 300 mM potassium phosphate buffer buffer, pH=6.5, containing NaCl (100 mM), DMSO (3% v:v), glucose (200 mM), and glycerol (10% v:v), 40 mM of 2-isobutyl-3-ketoketoglutarate diethyl ester was added along with 100 mg of lyophilized KRED 1001 and 30 mg of glucose dehydrogenase. The reaction mixture was incubated for 48 hours at 37 C. Gas chromatographic analysis showed that the yield of hydroxy diester product was greater than 80%. The product was isolated by extraction of the reaction mixture with ethyl acetate and purification using silica gel chromatography. Isolated yield was in the range of 85–95%.
출처 및 근거
| DOI | 10.6084/m9.figshare.5104873.v1 |
|---|---|
| 특허 번호 | US07081535B2 |
| 출처 데이터셋 | uspto-grants-2006_07 |
| ORD ID | ord-cb2716e4893e47f5b420d1a47f407af7 |
| 출처 메모 | uspto-grants-2006_07 |