Carbon Dioxide → HCID 24779772, compound

HRID 1801439Notes

Reaction equation

HRID 1801439 reaction equation
[CH3:1]OC(F)(F)C(Cl)[Cl:5].CC1(C)S[C@@H:13]2[C@H:15](N[C:19]([CH2:21][C:22]3[CH:23]=[CH:24][CH:25]=[CH:26][CH:27]=3)=[O:20])[C:16](=[O:17])N2[C@H]1C([O-])=O.[K+].C[C@@H]1[O:38][C@@H:37]([O:39][C@H:40]2[C@H:45](O)[C@@H:44](O)[C@H:43](NC(N)=N)[C@@H:42](O)[C@@H:41]2[NH:53][C:54](N)=N)[C@H](O[C@@H:58]2O[C@@H](CO)[C@H](O)[C@@H:60](O)[C@@H:59]2[NH:68][CH3:69])[C@@]1(O)C=O.N[C@H:74]([C:80]([OH:82])=O)[CH2:75][CH2:76][C:77](=[O:79])N.[C:83](=O)=O>CS(C)=O>[CH3:75][CH:74]([N+:53]1([CH3:54])[C@@H:41]2[CH2:1][CH:40]([O:39][C:37]([CH:21]([C:22]3[CH:27]=[CH:26][CH:25]=[CH:24][CH:23]=3)[CH2:19][OH:20])=[O:38])[CH2:45][C@H:44]1[CH2:43][CH2:42]2)[CH3:80].[CH3:83][C:59]([NH:68][CH2:69][C@H:80]([OH:82])[C:74]1[CH:75]=[CH:76][C:77]([O-:79])=[C:15]([CH2:16][OH:17])[CH:13]=1)([CH3:58])[CH3:60].[ClH:5]

Participants

7

Reactant 5

HCID 280Carbon DioxideHCID 4116MethoxyfluraneHCID 5961L-GlutamineHCID 23664709Penicillin G PotassiumHCID 178484872HCID 178484872, compound

Product 1

HCID 24779772HCID 24779772, compound

Reagents, catalysts & solvents 1

HCID 679Dimethyl SulfoxideSolvent

Procedure

For isolated mouse aortic rings preparations, male C57 black mice (5-7 weeks of age) were anesthetized with methoxyflurane and sacrificed by exsanguination. The thoracic aorta was dissected and cut into cylindrical segments of three millimeters in length. Using a sterile 48-well tissue culture plate, each ring was incubated in a total volume of one ml with either peptides or vehicle (DMSO) diluted in DMEM supplemented with penicillin (100 units/ml), streptomycin (100 μg/ml) and L-glutamine (1 mM). The plate was incubated with 5% CO2. Following incubation with the peptides (one, six, twelve or twenty hours), rings were washed in oxygenated (95% O2-5% CO2) Krebs-Henseleit bicarbonate buffer solution (KITS) of the following composition (NaCl-118.3 mmol/L, KCl-4.7 mmol/L, CaCl2-2.5 mmol/L, MgSO4-1.2 mmol/L, KH2PO4-1.2 mmol/L; NaHCO3-25 mmol/L, glucose-5.6 mmol/L at 37° C. with ibuprofen (10 μM). The rings were suspended by two tungsten wires (25 mm thickness) inserted into the lumen and mounted in a vessel myograph system (five ml chamber, Kent Scientific). The mouse aortas were submitted to a resting tension of 1.5 grams and isometric tension was recorded using a force transducer coupled to a MacLab data acquisition system. Following a sixty minute equilibration period, with frequent washes, the rings were precontracted with phenylephrine (PE) (10 μM; sub maximal concentration) and concentrations of Ach (1 nM-10 μM), sodium nitroprusside (10 μM) or Nitro-L-Arginine Methyl Ester (L-NAME, 100 mM) were injected at the plateau of the PE contraction. In some experiments concentration-response curves to the contractile effects of PE were monitored.

Workup

  1. customFor isolated mouse aortic rings preparations
  2. custommale C57 black mice (5-7 weeks of age)
  3. additiondiluted in DMEM
  4. washwere washed
  5. customin oxygenated (95% O2-5% CO2) Krebs-Henseleit bicarbonate buffer solution (KITS) of the following composition (NaCl-118.3 mmol/L, KCl-4.7 mmol/L, CaCl2-2.5 mmol/L, MgSO4-1.2 mmol/L, KH2PO4-1.2 mmol/L
  6. customat 37° C.
  7. custommounted in a vessel
  8. waitFollowing a sixty minute
  9. concentrationIn some experiments concentration-response curves to the contractile effects of PE

Sources & evidence

ORD record
ord-9835b14ca2a84d578b1d1e6084277d57
Source dataset
uspto-grants-2013_01
Patent number
US08349798B2

Reaction notes

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