反应详情

To a 1 dram vial was added 3-(2-(4-fluorophenyl)-3-(methylcarbamoyl)benzofuran-5-yl)-4-methylbenzoic acid (30.3 mg, 0.075 mmol) in DMF (1 mL) along with N-ethyl-N-isopropylpropan-2-amine (0.065 mL, 0.375 mmol), 1-(1,8-naphthyridin-2-yl)cyclopropanamine, HCl (31.1 mg, 0.113 mmol) and finally HATU (2-(3H-[1,2,3]triazolo[4,5-b]pyridin-3-yl)-1,1,3,3-tetramethyl isouronium hexafluorophosphate(V), (114 mg, 0.300 mmol). The vial was sealed and the tan mixture shaken overnight at room temperature. The crude product was purified using a Shimadzu preparative HPLC employing acetonitrile/water/trifluoroacetic acid where solvent A was 10% acetonitrile/90% water/0.1% trifluoroacetic acid and solvent B was 10% water/90% acetonitrile/0.1% trifluoroacetic acid with a Waters Sunfire 5 μm C18 19×150 mm column at a gradient of 20-100% B and a flow rate of 25 mL/min. over 22 minutes with a 8 minute hold. The solvent was removed giving 45.0 mgs (87% yield) of 5-(5-(1-(1,8-naphthyridin-2-yl)cyclopropylcarbamoyl)-2-methylphenyl)-2-(4-fluorophenyl)-N-methylbenzofuran-3-carboxamide, TFA as a tan solid. The LC/MS data was obtained on a Shimadzu analytical LC/Micromass Platform LC (ESI+) at 220 nm using the following set of conditions: Phenomenex Luna 3 μm C18, 2×50 mm column, with a gradient of 0-100% B (B=90% HPLC grade acetonitrile/0.1% trifluoroacetic acid/10% HPLC grade water), (A=90% HPLC grade water/0.1% trifluoroacetic acid/10% HPLC grade acetonitrile), in 4 minutes with a 1 minute hold at a rate of 0.8 mL/minute. HPLC purity was determined using a Shimadzu analytical LC at 254 nm and 256 nm with a Waters Sunfire C18 3.5 μm 4.6×150 mm column employing water/acetonitrile/0.1% trifluoroacetic acid with a gradient of 10-100% B (B=95% HPLC grade acetonitrile/0.1% trifluoroacetic acid/5% HPLC grade water), (A=95% HPLC grade water/0.1% trifluoroacetic acid/5% HPLC grade acetonitrile), in 10 minutes with a 10 minute hold at a rate of 1 mL/minute. The HPLC purity was then confirmed with an orthogonal solvent system and column using a Shimadzu analytical LC with a Xbridge Phenyl C18 3.0 μm 4.6×150 mm column employing water/methanol/10 mM ammonium bicarbonate with a gradient of 10-100% B (B=95% HPLC grade methanol/10 mM ammonium bicarbonate/5% HPLC grade water), (A=95% HPLC grade water/10 mM ammonium bicarbonate/5% HPLC grade methanol), in 10 minutes with a 10 minute hold at a rate of 1 mL/minute. 1H NMR (400 MHz, THF-d8) δ ppm 1.44 (q, J=4.18 Hz, 2 H), 1.90-1.98 (m, 2 H), 2.30 (s, 3 H), 2.89 (d, J=4.77 Hz, 3 H), 7.18-7.26 (m, 2 H), 7.29 (dd, J=8.28, 1.76 Hz, 1 H), 7.34-7.39 (m, 1 H), 7.50 (dd, J=8.03, 4.52 Hz, 2 H), 7.57 (d, J=8.53 Hz, 1H), 7.69 (d, J=1.25 Hz, 1H), 7.74 (d, J=8.53 Hz, 1 H), 7.85-7.91 (m, 2H), 8.07-8.14 (m, 2 H), 8.18 (d, J=8.53 Hz, 1 H), 8.34 (dd, J=8.03, 1.76 Hz, 1 H), 8.73 (s, 1 H), 9.04 (dd, 1 H). LCMS rt=1.430 min., m/z 571.3 (M+H), 98.3% purity. HPLC (Sunfire C18) rt=6.931 min, 99.4% purity and (XBridge Phenyl C18) rt=11.521 min., 100% purity.

1. customThe vial was sealed
2. customThe crude product was purified
3. waita flow rate of 25 mL/min. over 22 minutes with a 8 minute hold
4. customThe solvent was removed