Cholesterol → HCID 131730175, compound

Participants

Reaction Steps

Fluorescein isothiocyanate (10 mg) was dissolved in 1 ml anhydrous DMSO and 20 μl of the FTIC solution was added to 1 ml of cholesterol-coated iron oxide-lipoprotein (0.5 mg/ml Fe) in 500 mM carbonate buffer solution (pH 9.5). The reaction mixture was reacted at room temperature for 1 hr and dialyzed in a phosphate buffer solution (PBS) to obtain FITC-cholesterol-coated iron oxide-lipoprotein nanoparticles. 200 μl of FITC-cholesterol-coated iron oxide-lipoprotein were injected via the tail vein of mice. FIG. 5 shows the fluorescent signal in the blood samples that were obtained serially over a time over 220 min after injection of the particles into the tail vein. The signals were normalized to the first time point obtained within 2 min of injection. The graph suggests a circulation half-life of about 30 min for a cholesterol-coated iron oxide-lipoprotein particle with a size of 97 nm.

Provenance & Evidence