Fluorescein 5-isothiocyanate → HCID 131730176, compound

HRID 1808680Notes

Reaction equation

[CH:1]1[C:6]([N:7]=[C:8]=[S:9])=[CH:5][C:4]2[C:10]([O:12][C:13]3([C:23]4[CH:24]=[CH:25][C:26]([OH:28])=[CH:27][C:22]=4[O:21][C:15]4[CH:16]=[C:17]([OH:20])[CH:18]=[CH:19][C:14]3=4)[C:3]=2[CH:2]=1)=[O:11].B([O-])([O-])[O-]>>[CH:1]1[C:6]([N:7]=[C:8]=[S:9])=[CH:5][C:4]2[C:10]([O:12][C:13]3([C:14]4[CH:19]=[CH:18][C:17]([OH:20])=[CH:16][C:15]=4[O:21][C:22]4[CH:27]=[C:26]([OH:28])[CH:25]=[CH:24][C:23]3=4)[C:3]=2[CH:2]=1)=[O:11].[CH:1]1[C:6]([N:7]=[C:8]=[S:9])=[CH:5][C:4]2[C:10]([O:12][C:13]3([C:14]4[CH:19]=[CH:18][C:17]([OH:20])=[CH:16][C:15]=4[O:21][C:22]4[CH:27]=[C:26]([OH:28])[CH:25]=[CH:24][C:23]3=4)[C:3]=2[CH:2]=1)=[O:11]

Participants

Reactant 2

HCID 18730Fluorescein 5-isothiocyanate

HCID 26574Borate

Product 1

HCID 131730176HCID 131730176, compound

Procedure

An HbA1C immunoassay was carried out on a nitrocellulose substrate (5.0 μm pore), on which was placed two 4 mm wide capture bands. The first band contained an HbA1C agglutinator (a mimic of the analyte HbA1C; 1 mg/mL in PBS, pH 7.4). The second band contained a monoclonal anti-FITC antibody (3 mg/mL in 0.05 borate, pH 8.5).

Sources & evidence

ORD record: ord-3f88d9becd2a4d7eb22f6f5158166fa5
Source dataset: uspto-grants-2013_01
DOI: 10.6084/m9.figshare.5104873.v1
Patent number: US08361782B2

Reaction notes

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