Sulfuric Acid → HCID 135931635, compound

Participants

Reaction Steps

Trifluoroacetic acid (1.5 ml) and conc. Sulphuric acid (1.5 ml) were added to 4-{[3-(3-cyano-4-hydroxy-phenyl)-imidazo[1,2-a]pyrazin-8-ylamino]-methyl}-benzenesulfonamide (0.093 g, 0.2 mmol) and the resulting solution was stirred at ambient temperature for 24 hours. The reaction mixture was neutralized with conc. Aqueous sodium carbonate solution and extracted with ethyl acetate (2×50 mL). The organic layers were combined and dried with magnesium sulphate, filtered and concentrated in vaccuo. The crude material was taken up in DMSO and purified by prep HPLC. The product, 2-hydroxy-5-[8-(4-sulfamoyl-benzylamino)-imidazo[1,2-a]pyrazin-3-yl]-benzamide, is obtained as a white solid (4.2 mg, 5%) (LC-MS m/z 439 [M+H]+).

Provenance & Evidence