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HRID1813888

反应详情

EQUATION

反应方程式

HRID 1813888 的结构方程式

REACTANTS

反应物

5
结构：OCCSCCO
HCID5447

2,2'-Thiobisethanol

C4H10O2S

结构：CCCCCCCCCCCCOCCO
HCID24750

2-(Dodecyloxy)ethanol

C14H30O2

结构：CC(C)(C)CC(C)(C)c1ccc(OCCOCCO)cc1
HCID24775

4-tert-Octylphenol diethoxylate

C18H30O3

结构：C[C@H](CCC(=O)NCCC[N+](C)(C)CC(O)CS(=O)(=O)[O-])[C@H]1CC[C@H]2[C@@H]3[C@H](O)C[C@@H]4C[C@H](O)CC[C@]4(C)[C@H]3C[C@H](O)[C@]12C
HCID122145

3-(Dimethyl{3-[(3,7,12,24-tetrahydroxycholan-24-ylidene)amino]propyl}azaniumyl)-2-hydroxypropane-1-sulfonate

C32H58N2O8S

结构：CCCCCCCCCCCCOS(=O)(=O)[O-].[Na+]
HCID3423265

Sodium Lauryl Sulfate

C12H25NaO4S

PRODUCTS

生成物

2
结构：C[C@H](CCC(=O)O)[C@H]1CC[C@H]2[C@@H]3CC[C@@H]4C[C@H](O)CC[C@]4(C)[C@H]3C[C@H](O)[C@]12C
HCID222528

Deoxycholic acid

C24H40O4

结构：CCCCCCCCCCCCOS(=O)(=O)[O-].[Na+]
HCID3423265

Sodium Lauryl Sulfate

C12H25NaO4S

PROCEDURE

实验过程

The FIG. 7 shows the results. The samples were loaded on the gels in the following order (from left to right) lane-1 control sample, lane 2-3 thiodiglycol, lane 4-5 Tween-20, lane 6-7 NP-40, lane 8-9 Brij-35, lane 10 SDS, lane 11 CHAPSO, 12-13 Triton-X100, lane 14 CHAP lane 15 control. The electrophoresis results shows (after a comparison of several typical runs) that, with exception of SDS and de-oxycholate treated protein samples, most protein samples containing detergent showed batch to batch variation in protein precipitation yield. The protein precipitation yield depended on the techniques used for harvesting the precipitated protein. There were occasions when the precipitation yield of duplicate samples varied between 100% to 50%. Protein samples containing SDS invariably showed substantially 100% yield in multiple tests.