Hydrochloric Acid → Adenosylhomocysteine

Participants

Reaction Conditions

Reaction Steps

L-Homocysteine sodium salt solution (850 Kg), prepared as described above, was cooled to 10° C. and dil.hydrochloric acid (1:1; 100 Lit) was added til the pH became 11.3 to 11.5. To this solution, 5′-Chloromethyl adenosine hydrochloride (135 Kg) and potassium iodide (13.5 Kg) were added and stirred at 70-80° C. for 5 h. The reaction was monitored by HPLC and cooled to 15° C. Dil.hydrochloric acid (1:1; 200 Lit) was added to bring down the pH to 2.0. Activated carbon (10 Kg) was added, stirred for 30 min and filtered. To the mother liquor, ammonia solution (15%) was added to set the pH to 6.0-6.2. The precipitate was stirred at 20° C. for 24 h and filtered. The solid was washed two times with water (200 Lit). The wet cake was dried at 40° C. under vacuum.

Provenance & Evidence