HCID 58649722, compound → HCID 58649720, compound

Participants

Reaction Steps

A solution of 4D (100 mg, 0.156 mmol) in TFA (1.2 ml) was stirred at rt for 5 min. The solution was concentrated in vacuo and the residue was purified by a Shimadzu auto prep HPLC, employing 30% to 100% 10 min gradient, 6 min 100% holding elution with 0.1%TFA in MeOH-water solvent system, 220 nM detection, 20 mL/min elution with a YMC ODS S5 20×100 mm column to give 4E (45 mg, 53%). Analytical HPLC retention time=2.93 min. (YMC S5 ODS column 4.6×50 mm, 10–90% aqueous methanol over 4 minutes containing 0.2% phosphoric acid, 4 mL/min, monitoring at 220 nm) and a LC/MS M++1=541.

Provenance & Evidence