6-Aminohexanoic Acid → Theophylline

Participants

Reaction Steps

1 mg of theophylline-8-butanoic acid (Sigma) was dissolved in 2 ml of a 0.1 M MES buffer solution. Thereafter, 3.6 mg of WSC hydrochloride and 2.5 mg of NHS were added thereto, and the mixture was stirred at room temperature for 15 minutes. Thereafter, 2.0 mg of ε Amino Caproic Acid (Sigma) was dissolved in 200 μl of 0.1 M MES, and the obtained solution was added to the above reaction product. The obtained mixture was reacted at room temperature for 2 hours. 100 μl of 1M Tris buffer solution (pH 7.5) was added to the mixture, and the reaction was terminated. Thereafter, the obtained reaction product was adsorbed to a column filled with 8 g of ODS silica (YMC-ODS-AQ 120A), followed by elution with a 30% methanol aqueous solution. The eluant was concentrated and then purified by medium-pressure separation chromatography (YAMAZEN Ultrapack ODS-S-4OB).

Provenance & Evidence