反应详情

As seen from Table 4, when L-leucine, L-arginine hydrochloride, and glycine were added to the medium in an amount of each 0.25%, the yield of leupeptin in 72 hours of cultivation was insignificant, whereas when each 0.5 to 1.25% of said amino acids were added, the yield of leupeptin in the same period of cultivation was as high as 2,560 to 4,370 mcg/ml. However, when the amount of said amino acids was increased to each 1.50%, only insignificant amount of leupeptin was produced. These results confirmed that a high yield of leupeptin is obtained specifically when each 0.5 to 1.25% of L-leucine, L-arginine (as hydrochloride), and glycine is added to the medium.

1. additionwere added to the medium in an amount of each 0.25%
2. customwas produced