Hydrochloric Acid → HCID 131724508, compound

Participants

Reaction Steps

632.7 mg distearoylphosphatidylglycerol sodium salt (Avanti Polar Lipids, Birmingham, Ala.) was dissolved in 4 ml of an equivolume solution of chloroform and methanol at 65° Centigrade. 300 ul 2.5 M HCl was added to the solution. 375.9 mg Amphotericin B (Squibb Pharmaceuticals, New Brunswick, N.J.) was first suspended in 4.0 ml of equivolume solution of chloroform and methanol, and then the suspension was added to the acidified DSPG solution. The Amphotericin B—DSPG lipophilic complex was formed with heating at 65° C. for several minutes yielding an orange solution of the dissolved Amphotericin B complex with a pH of approximately 1.5. The concentration of Amphotericin B was approximately 45 mg/ml.

Provenance & Evidence