Sulfate Ion → HCID 178463689, compound

Participants

Reaction Steps

Detection and Quantification of Amphetamine using a Reactive Coating on Silver Foil: Silver foil was roughened and etched (Example 1d) followed by dip-coating in an CH2Cl2 solution of MNA/amphetamine containing 3 ppm of a CH2Cl2 solution of pentachlorothiolphenol (PCTP). The PCTP was used as an internal standard for quantification purposes. The coated foil surfaces were rinsed with ethanol to remove unbound MNA/amphetamine and PCTP. The MNA/amphetamine was synthesized by dissolving 363 mg amphetamine sulfate in 10 mL of an aqueous saturated solution of K2CO3 to neutralize the sulfate. The freebase drug was then extracted from the aqueous solution using CH2Cl2 followed by addition of 155 mg 2-mercaptonicotinic acid (MNA) and 206 mg dicyclohexylcarbodiimide (DCC). The reaction was gently refluxed overnight under a nitrogen atmosphere. The MNA/amphetamine reaction product was transferred to a separatory funnel with CH2Cl2 and washed with an aqueous saturated solution of NaHCO3 to neutralize unreacted MNA, followed by a wash with H2O and 10% HCI to neutralize unreacted freebase and DCC. Solvent was removed under reduced pressure. The product N-(1-methyl-2-phenylethyl)-2-mercaptopyridine-3-carboxamide (MNA/amphetamine) was re-dissolved in CH2Cl2 to form standard solutions in the concentration range of 0 ppm to 500 ppm. SERS spectra were obtained from the MNA/amphetamine/PCTP coated silver foil surfaces.

Provenance & Evidence