反应详情

To a solution of (±)-trans-tert-butyl 2-[4-methoxy-2-(trifluoromethyl)phenyl]-5,6,8a,9,11,11a-hexahydro-4H-pyrido[3,2,1-ij]pyrrolo[3,4-c]quinoline-10(8H)-carboxylate (160 mg, 0.33 mmol) in 5 mL of methylene chloride was added 1 mL of trifluoroacetic acid. The mixture was allowed to stir at ambient temperature for 2 h and then was concentrated in vacuo. The residue was purified by preparative HPLC (C18 reverse phase column, elution with a H2O/CH3CN gradient with 0.5% TFA). Product-containing fractions were combined, concentrated and partitioned between chloroform and saturated aqueous sodium carbonate. The organics were washed with brine, dried (K2CO3) and concentrated in vacuo. The residue (30 mg, 0.077 mmol) was dissolved in ethanol and ether and there was added 2M HCl in ether (0.077 mL, 0.15 mmol). The solvent was decanted and the remaining solid was triturated twice with ether and was dried in vacuo to afford 20 mg (57%) of the title compound of EXAMPLE 7 as an off white powder. 1H NMR (d6-dmso): δ 9.40 (broad s, 2H), 7.29-7.18 (m, 3H), 6.71 (s, 1H), 6.58 (s, 1H), 3.82 (s, 3H), 3.57-3.49 (m, 1H), 3.40-3.18 (m, 5H), 2.93-2.80 (m, 3H), 2.70-2.62 (m, 2H), 2.16-2.06 (m, 1H), 1.95-1.88 (m, 1H), 1.85-1.78 (m, 1H). LRMS (ES)+: 389.2 (M+H)+.

1. concentrationwas concentrated in vacuo
2. customThe residue was purified by preparative HPLC (
3. washC18 reverse phase column, elution with a H2O/CH3CN gradient with 0.5% TFA)
4. concentrationconcentrated
5. custompartitioned between chloroform and saturated aqueous sodium carbonate
6. washThe organics were washed with brine
7. dry with materialdried (K2CO3)
8. concentrationconcentrated in vacuo
9. customThe solvent was decanted
10. customthe remaining solid was triturated twice with ether
11. customwas dried in vacuo