HCID 21988071, compound → Biotin

HRID 1840226Notes

Reaction equation

HRID 1840226 reaction equation
[OH:1][C:2]([CH2:4][CH2:5][CH2:6][CH2:7][C@H:8]1[C@@H:16]2[C@@H:11]([NH:12][C:13]([NH:15]2)=[O:14])[CH2:10][S:9]1)=[O:3].CCN(CCOC1C=CC(CC2C=CC=CC=2)=CC=1)CC.Cl.C(O)C(O)C.OCC(CO)O>O>[OH:3][C:2]([CH2:4][CH2:5][CH2:6][CH2:7][C@H:8]1[C@@H:16]2[C@@H:11]([NH:12][C:13]([NH:15]2)=[O:14])[CH2:10][S:9]1)=[O:1]

Participants

4

Reactant 2

HCID 21988071HCID 21988071, compoundHCID 131724297HCID 131724297, compound

Product 1

HCID 171548Biotin

Reagents, catalysts & solvents 1

HCID 962WaterSolvent

Conditions

Temperature
80 °C

Procedure

To a mixture of phosphatidylserine (5 mg, Avanti) and biotin-DPPE (0.6 mg, Pierce) in a clean vial was added 5% propyleneglycol-glycerol in water (1 mL). The dispersion was heated to 80° C. for 5 minutes and then cooled to ambient temperature. The head space was then flushed with perfluorobutane and the vial shaken in a cap-mixer for 45 seconds. After centrifugation the infranatant was removed and the microbubbles formed washed extensively with water.

Workup

  1. temperaturecooled to ambient temperature
  2. customThe head space was then flushed with perfluorobutane
  3. customa cap-mixer for 45 seconds
  4. customAfter centrifugation the infranatant was removed
  5. customthe microbubbles formed
  6. washwashed extensively with water

Sources & evidence

ORD record
ord-322eea0b3586438c93669b3edd2234a4
Source dataset
uspto-grants-2004_01
Patent number
US06680047B2

Reaction notes

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