反应详情

In N,N-dimethylformamide (5 ml) was dissolved 3-(7-methoxy-2-oxoquinoxalin-1(2H)-yl)propanal (Reference Example 8,200 mg, 0.86 mmol). 6-[(4R)-4-Amino-2-oxopyrrolidin-1-yl]-2H-pyrido[3,2-b][1,4]oxazin-3(4H)-one (synthesized using [(3R)-5-oxopyrrolidin-3-yl]carbamic acid tert-butyl ester synthesized with reference to WO2004/22536 in the same manner as in Reference Examples 20, 21 and 22; 262 mg, 1.03 mmol) was added thereto and subsequently acetic acid (65 mg, 1.12 mmol) was added. The obtained mixture was stirred at room temperature for 1 hour, cooled on ice and sodium triacetoxyborohydride (237 mg, 1.12 mmol) was added thereto. After stirring at room temperature for 2 hours, sodium hydrogen carbonate aqueous solution was added thereto and the mixture was extracted with ethyl acetate. The obtained organic layer was washed with water and a saturated sodium chloride solution, dried over anhydrous sodium sulfate and the solvent was removed under reduced pressure. The obtained crude product was purified by thin layer chromatography (dichloromethane/methanol) to yield 80 mg (20%) of the title compound.

1. customsynthesized with reference to WO2004/22536 in the same manner as in Reference Examples 20, 21 and 22
2. addition262 mg, 1.03 mmol) was added
3. additionwas added
4. stirringAfter stirring at room temperature for 2 hours
5. extractionthe mixture was extracted with ethyl acetate
6. washThe obtained organic layer was washed with water
7. dry with materiala saturated sodium chloride solution, dried over anhydrous sodium sulfate
8. customthe solvent was removed under reduced pressure
9. customThe obtained crude product
10. customwas purified by thin layer chromatography (dichloromethane/methanol)