Phenol → Phenol chloroform

HRID 1867208Notes

Reaction equation

HRID 1867208 reaction equation
[C:1]1([OH:7])[CH:6]=[CH:5][CH:4]=[CH:3][CH:2]=1.[CH:8]([Cl:11])([Cl:10])[Cl:9]>O>[C:1]1([OH:7])[CH:6]=[CH:5][CH:4]=[CH:3][CH:2]=1.[CH:8]([Cl:11])([Cl:10])[Cl:9]

Participants

4

Reactant 2

HCID 996PhenolHCID 6212Chloroform2×

Product 1

HCID 18475210Phenol chloroform

Reagents, catalysts & solvents 1

HCID 962WaterSolvent3×

Procedure

Sterile water was added to the sample DNA liquid to a total of 100 μl, and 50 μl of TE saturated phenol (Nippon Gene) and 50 μl of chloroform (Wako) were added and agitated for 1 to 2 minutes in a tube mixer to inactivate the protein. After 5 minutes of centrifugation at 14,000 rpm, the water layer alone was transferred to a 1.5 ml tube. 100 μl of chloroform was added, followed by centrifugation for 5 minutes at 14,000 rpm in a vortex mixer. The water layer alone was transferred to a 1.5 ml tube.

Workup

  1. additionwere added
  2. waitAfter 5 minutes of centrifugation at 14,000 rpm
  3. waitfollowed by centrifugation for 5 minutes at 14,000 rpm in a vortex mixer

Sources & evidence

ORD record
ord-3e43807b009d41fda454127f700a0404
Source dataset
uspto-grants-2009_12
Patent number
US07632676B2

Reaction notes

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