Phosphate → ethyl N-Boc-2-amino-3-cyclohexyl-3-hydroxypropionate

HRID 1873445Notizen

Reaktionsgleichung

Reaktionsgleichung von HRID 1873445
[C:1]([NH:8][CH:9]([C:15]([CH:17]1[CH2:22][CH2:21][CH2:20][CH2:19][CH2:18]1)=[O:16])[C:10]([O:12][CH2:13][CH3:14])=[O:11])([O:3][C:4]([CH3:7])([CH3:6])[CH3:5])=[O:2].P([O-])([O-])([O-])=O.C1N=C(N)C2N=CN([C@@H]3O[C@H](COP(OP(OC[C@H]4O[C@@H](N5C=C(C(N)=O)CC=C5)[C@H](O)[C@@H]4O)(O)=O)(O)=O)[C@@H](O)[C@H]3O)C=2N=1>C(OCC)(=O)C>[C:1]([NH:8][CH:9]([CH:15]([CH:17]1[CH2:22][CH2:21][CH2:20][CH2:19][CH2:18]1)[OH:16])[C:10]([O:12][CH2:13][CH3:14])=[O:11])([O:3][C:4]([CH3:7])([CH3:5])[CH3:6])=[O:2]

Komponenten

5

Edukt 3

HCID 1061PhosphateHCID 439153NadhHCID 67424848ethyl N-Boc-2-amino-3-cyclohexyl-3-oxopropionate2×

Produkt 1

HCID 67424734ethyl N-Boc-2-amino-3-cyclohexyl-3-hydroxypropionate

Reagenzien, Katalysatoren, Lösungsmittel 1

HCID 8857Ethyl AcetateLösungsmittel

Versuchsvorschrift

The reducing activity against ethyl N-Boc-2-amino-3-cyclohexyl-3-oxopropionate was calculated in the following manner. First, an appropriate amount of a crude enzyme solution and 100 mM phosphate buffer (pH 6.5) were added to a test tube to make a total volume of 0.5 ml. Further, 0.25 mg of ethyl N-Boc-2-amino-3-cyclohexyl-3-oxopropionate and 1 mg of NADH were added, and the reaction was allowed to proceed at 30° C. for 2 hours with shaking. After the reaction, 1 ml of ethyl acetate was added and extraction was carried out. The quantitation of the product ethyl N-Boc-2-amino-3-cyclohexyl-3-hydroxypropionate was carried by capillary gas chromatography (column: GL Sciences InertCAP 5 (ID 0.25 mm×30 m), column temperature: 200° C.; carrier gas: helium (70 kPa), detection: FID). The enzyme activity was calculated from the amount of the ethyl N-Boc-2-amino-3-cyclohexyl-3-hydroxypropionate formed. The activity such that 1 μmol of ethyl N-Boc-2-amino-3-cyclohexyl-3-hydroxypropionate is formed in 1 minute under the reaction conditions mentioned above is defined as 1 unit.

Aufarbeitung

  1. additionwas added
  2. extractionextraction
  3. customwas carried by capillary gas chromatography (column: GL Sciences InertCAP 5 (ID 0.25 mm×30 m), column temperature: 200° C.; carrier gas: helium (70 kPa), detection: FID)
  4. customformed

Quellen und Nachweise

ORD-Eintrag
ord-cd42fd9c26f04f918c2077d9d7b4777e
Quelldatensatz
uspto-grants-2012_11
Patentnummer
US08304216B2

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