反应详情

温度

0 °C

200 mg (0.57 mmol) of 2-[3-(2-fluorobenzyl)-1H-pyrazolo[4,3-b]pyridin-1-yl]pyrimidine-4,5,6-triamine from example 8 are dissolved in 10 ml of pyridine. The solution is cooled to 0° C., and 81 mg (0.86 mmol) of methyl chloroformate are added. The reaction mixture is stirred at RT overnight and then concentrated in vacuo, mixed with ethyl acetate and washed twice with water and with saturated sodium chloride solution. The organic phase is dried over sodium sulfate and concentrated in vacuo. The residue is purified by preparative HPLC to result in 125 mg (54% of theory) of the title compound as a pale beige solid.

1. concentrationconcentrated in vacuo
2. additionmixed with ethyl acetate
3. washwashed twice with water and with saturated sodium chloride solution
4. dry with materialThe organic phase is dried over sodium sulfate
5. concentrationconcentrated in vacuo
6. customThe residue is purified by preparative HPLC