Sodium Hydroxide → HCID 59529506, compound

Participants

Reaction Steps

To a mixture of 4-({cyclohexyl[5-(3,6-dihydro-2H-thiopyran-4-yl)-2-ethylthiophen-3-yl]methyl}amino)benzoic acid (246 mg) synthesized above, ethyl β-alaninate hydrochloride (98.8 mg), 1-hydroxybenzotriazole monohydrate (98.5 mg) and triethylamine (90 μL) in N,N-dimethylformamide (10 mL) was added 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (123 mg), and the mixture was stirred at room temperature for 8 hr. Saturated aqueous ammonium chloride solution was added to quench the reaction, and the mixture was extracted with ethyl acetate. The extract was washed with saturated brine, dried over magnesium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (ethyl acetate:hexane=1:1, volume ratio) to give a brown oil. To a mixture of the obtained oil, tetrahydrofuran (5 mL) and ethanol (5 mL) was added 1N aqueous sodium hydroxide solution (1 mL), and the mixture was stirred at room temperature for 2.5 hr, and concentrated under reduced pressure. The residue was dissolved in water (10 mL), and 1N hydrochloric acid (1 mL) was added at 0° C. The resulting precipitate was collected by filtration to give the title compound (145 mg, 47%) as a brown solid.

Provenance & Evidence