Sodium Hydroxide → HCID 59294530, compound

Participants

Reaction Steps

To a suspension of 3-(4-{4-[2-(2,5-dichloro-phenoxy)-pyridine-3-carbonyl]-3,4-dihydro-2H-quinoxaline-1-sulfonyl}-phenyl)-propionic acid methyl ester (36 mg, 0.058 mmol, 1.0 equiv) in ethanol (1 mL) was added a 1 M solution of sodium hydroxide (120 μL, 0.12 mmol, 2.0 equiv; [CAS RN 1310-73-2]) and the reaction mixture stirred at rt over night. Removal of the solvent under reduced pressure and purification by preparative HPLC on reversed phase (Xterra® PrepMSC 18, 5 μm, 19×50 mm column equipped with a Gilson Liquid Handler 215 autosampler, two Rainin Dynamax® SD-300 pumps, a Sedex ELSD 75 lightscatter and a Dionex UVD 340S UV detector) eluting with a gradient of acetonitrile/water provided 4.5 mg (13%) of the title compound as a light yellow powder. MS (ISP): 612.1 [M+H]+.

Provenance & Evidence