HuagongsheHuagongshe Chemistry Print Report · For research reference only
Nadide (JAN/USAN/INN) → Nadh
Participants
| Role | Name | HCID | Charge |
|---|---|---|---|
| Reactant | Nadide (JAN/USAN/INN) | HCID 5893 | — |
| Reactant | (2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanal | HCID 107526 | — |
| Solvent | Tris(hydroxymethyl)aminomethane | HCID 6503 | — |
| Product | Nadh | HCID 439153 | — |
Reaction Steps
A mixture of substrate (5 mM), oxidized cofactor NAD+ (100 μM), glucose (20 mM) in 50 mM Tris buffer pH 7.5 (0.8 ml) was admixed with (D)-GDH (10 u), after the enzyme (100-200 μg) had been added, and the reaction was carried out at 30° C. (140 rpm) for 48 hours.
Provenance & Evidence
| DOI | 10.6084/m9.figshare.5104873.v1 |
|---|---|
| Patent number | US08313923B2 |
| Source dataset | uspto-grants-2012_11 |
| ORD ID | ord-b1c50b44bdaa455398b5cf7ec2ccbde9 |
| Source note | uspto-grants-2012_11 |