Methanaminium, N-((dimethylamino)(3H-1,2,3-triazolo(4,5-b)pyridin-3-yloxy)methylene)-N-methyl-, hexafluorophosphate(1-) (1:1) → HCID 25253999, compound

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Reaction Steps

The title compound was synthesized from 4-(9-cyclohexyl-7,7-difluoro-5-methyl-6-oxo-6,7,8,9-tetrahydro-5H-pyrimido[5,4-b][1,4]diazepin-2-ylamino)-3-methoxybenzoic acid as described in the General procedure for amide bond synthesis using HATU and 1-N—Boc-hexahydro azepine 4-amine. The final compound was purified by reverse phase HPLC and basified to give the free base. Further, after washing with water (10 ml), t-butoxycarbonyl (Boc) protection group was removed using 40% TFA in dichloromethane (6 ml) and purified the product using preparative HPLC. 1H NMR (400 MHz, DMSO-d6) δ ppm 1.10-2.05 (m, 16 H) 2.88 (d, J=3.28 Hz, 1 H) 3.01 (br. s., 2 H) 3.31 (s, 3 H) 3.34 (br. s., 1 H) 3.94 (s, 3 H) 4.06 (t, J=13.26 Hz, 3 H) 4.46 (m, 1 H) 7.47 (d, J=8.1 Hz, 1 H) 7.50 (s, 1 H) 7.91 (s, 1 H) 8.21 (s, 1 H) 8.23-8.30 (m, 2 H). [M+H] calculated for C28H37F2N7O3, 558; found 558.

Provenance & Evidence