Water → alpha-Hydroxyacetophenone
Reaction equation
[CH3:1][C:2]([C:4]1[CH:9]=[C:8](Cl)[C:7](O)=[C:6](Cl)[CH:5]=1)=[O:3].[OH2:13]>>[OH:13][CH2:1][C:2]([C:4]1[CH:9]=[CH:8][CH:7]=[CH:6][CH:5]=1)=[O:3]Participants
3
Procedure
As a stock solution (5/5), 0.2 mM solution of 3,5-dichloro-4-hydroxyacetophenone was used. The solution was diluted with purified water to prepare 5-stage serial dilutions. To 0.02 ml of the solution in each concentration was mixed with 0.38 ml each of 0.1M citrate buffer solution (pH 5.4) containing 0.1% Triton X-100 and a buffer solution further supplemented with 0.3% BSA. Each absorbance was measured at 340 nm with a spectrophotometer to prepare the standard curve (FIG. 2).
Workup
- customto prepare 5-stage serial dilutions
- concentrationTo 0.02 ml of the solution in each concentration
- additionwas mixed with 0.38 ml each of 0.1M citrate buffer solution (pH 5.4)
- additioncontaining 0.1% Triton X-100
- customto prepare the standard curve (FIG. 2)
Sources & evidence
- ORD record
- ord-b686e376550f4bf7a03cb33c2ba70f4a
- Source dataset
- uspto-grants-1992_09
- Patent number
- US05151372
Reaction notes
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