HCID 66887685, compound → HCID 49854434, compound

Participants

Reaction Steps

Step-3: A solution of tert-butyl 3-((2-amino-3-carbamoylphenyl)carbamoyl)-7-((tert-butoxycarbonyl)amino)-3,4-dihydroisoquinoline-2(1H)-carboxylate (70 mg, 0.133 mmol) in acetic acid (2 ml) was heated to 80° C. and stirred for 2.5 h. The reaction mixture was concentrated, neutralized with aq. NaHCO3 and extracted with DCM. The DCM layer was washed with brine, dried over anhydrous sodium sulfate and evaporated. Purification of the crude product by preparative TLC using methanol/dichloromethane (1:15) mixture as eluent afforded tert-butyl 7-((tert-butoxycarbonyl)amino)-3-(4-carbamoyl-1H-benzo[d]imidazol-2-yl)-3,4-dihydroisoquinoline-2(1H)-carboxylate. MS (ES+): m/z 508.28 [M+H]+

Provenance & Evidence