Ammonium Chloride → HCID 178513486, compound

Participants

Reaction Steps

To a solution of 82.8 mg of 1-(3-chloro-2-fluorobenzoyl)-4-((6-(1H-pyrazol-3-ylamino)pyridin-2-yl)methyl)piperidine-4-carboxylic acid in 5 ml of chloroform were added 97 mg of ammonium chloride, 0.252 ml of triethylamine, 83 mg of hydroxybenzotriazole hydrate and 104 mg of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride at room temperature, followed by stirring the reaction mixture at room temperature overnight. The reaction mixture was poured into saturated aqueous sodium bicarbonate solution, and extracted with ethyl acetate. The resulting ethyl acetate layer was dried over anhydrous magnesium sulfate, and filtered. The filtrate was concentrated in vacuo. The resulting residue was purified by a preparative thin-layer chromatography (Kieselgel™ 60F254, Art5744 (Merck), chloroform/methanol=6/1) to give the title compound as a pale yellow solid.

Provenance & Evidence