Hydrochloric Acid → (R)-2-Acetamido-3-(4-cyanophenyl)propanoic acid

Participants

Reaction Steps

A mixture of DL-4-cyano-N-acetylphenylalanine (114.0 g), cobalt chloride(II) (0.63 g) and acylase Amano 15000 (Trademark) (6.27 g) in 1N sodium hydroxide aqueous solution (492 ml) was adjusted to pH=7.5 with 0.1N hydrochloric acid and 0.1N sodium chloride aqueous solution, and then was stirred at 33°-36° C. for 20 hours. The reaction mixture was adjusted to pH=1 with 10% hydrochloric acid, and was washed with ethyl acetate, and the resulting precipitate was collected by filtration. The precipitate was washed with 10% hydrochloric acid and water to give 4-cyano-N-acetyl-D-phenylalanine (32.00 g).

Provenance & Evidence