Methanol → 3'-O-Methyl-D-adenosine

Participants

Reaction Steps

10 g of adenosine was suspended in 2 l of 1 mmole methanol solution of tin chloride dihydrate. 500 ml of 0.4-0.5M 1,2-dimethoxyethane solution of diazomethane was added to the solution with stirring. After stirring for 2 hours at room temperature, the solution was evaporated to dryness under reduced pressure. The residue was purified by ion exchange column chromatography and recrystallized from ethanol to give 2'-O-methyladenosine (Compound 1) and 3'-O-methyladenosine (Compound 2).

Provenance & Evidence