Potassium Hydroxide → HCID 86741339, compound

Participants

Reaction Steps

The diformyl amide obtained in Example 2 (2 g) was hydrolyzed with 5% methanolic KOH (reflux, 1 hr). Dilution with water and extraction with ethyl acetate gave chromatographically pure 2-(3α,12α-dihydroxy-7-oxo-5β-cholan-24-amido)-2-methyl-1-propanol which was recrystallized from ethyl acetate (colorless prisms), melting at 229°-231° C. PMR (δ ppm): 0.70 (3H, s, 18--CH3), 1.09 (3H, d, J=6 Hz, 21--CH3), 1.16 (3H, s, 19--CH3), 1.52 (6H, s, --C(CH3)2 --), 3.65 (1H, m, 3β--H), 3.86 (2H, s, --CH2OH), 4.09 (1H, m, 12β--H), 7.54 (1H, s, --NH--).

Provenance & Evidence