Hydrochloric Acid → HCID 86651035, compound

Participants

Reaction Steps

To a solution of 40 mg 4-[(S)-2-{[5-(3,3-Dimethyl-2-oxo-butoxy)-1-phenyl-1H-pyrazole-3-carbonyl]-amino}-3-(2-ethoxy-3,4-dioxo-cyclobut-1-enylamino)-propionyl]-piperazine-1-carboxylic acid ethyl ester in 2 ml of THF, 0.2 ml of a 1 M aqueous NaOH solution was added. After stirring for 5 h at RT the reaction was acidified to pH 1 with diluted aqueous hydrochloric acid and extracted with DCM (3×50 ml). The combined organic phases were dried over MgSO4 and the solvents were removed under reduced pressure. The product was dissolved in water and lyophilized to yield a white solid.

Provenance & Evidence