(2-Hydroxyphenyl)acetic acid → N-[3-(4-chlorophenyl)pyrazolo[1,5-a]pyrimidin-2-yl]-2-(2-hydroxyphenyl)acetamide

Participants

Reaction Conditions

Reaction Steps

The product from Example 99A (100 mg, 0.41 mmol), 2-hydroxyphenylacetic acid (124 mg, 0.82 mmol), 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (157 mg, 0.82 mmol), 1-hydroxybenzotriazole hydrate (100 mg, 0.65 mmol) were combined with pyridine (8 mL) and stirred at 35° C. for 18 hours. The reaction mixture was concentrated under vacuum and purified by reverse-phase HPLC [Waters XBridge™ RP18 column, 5 μm, 30×100 mm, flow rate 40 mL/minute, 5-95% gradient of acetonitrile in buffer (0.1 M aqueous ammonium bicarbonate, adjusted to pH 10 with ammonium hydroxide)] to afford 30 mg (19%) of the title compound. 1H NMR (400 MHz, DMSO-d6) δ ppm 10.28-10.56 (bs, 1H), 9.09 (dd, J=6.9, 1.7 Hz, 1H), 8.62 (dd, J=4.1, 1.7 Hz, 1H), 7.70-7.73 (m, 2H), 7.30-7.34 (m, 2H), 7.11-7.16 (m, 2H), 6.72-6.81 (m, 2H), 6.65-6.72 (m, 1H), 3.57 (s, 2H); MS (ESI) m/z 379 (M+H)+; Anal. calculated for C20H15ClN4O2.0.35H2O: C, 62.37; H, 4.11; N, 14.55. Found C, 62.16; H, 3.87; N, 14.49.

Provenance & Evidence