Ammonia → HCID 68712548, compound

Participants

Reaction Steps

337 mg (1.0 mmol) of 6-(5-oxo-4-pyridin-3-yl-2,5-dihydro-1H-pyrazol-1-yl)pyridine-3-carbonyl chloride are suspended in 5 ml of dioxane. Slowly and with stirring, 4 ml of a 5 N solution of ammonia in dioxane are then added, and the mixture is stirred at RT for 16 h. The mixture is then concentrated, and the residue is taken up in DMSO and purified by preparative HPLC (RP18 column; mobile phase: acetonitrile/water gradient with 0.1% TFA added to the water). The product-containing fractions are combined and concentrated on a rotary evaporator. The residue is dissolved in 1 N hydrochloric acid, and the solution is then lyophilized.

Provenance & Evidence