Hydrochloric Acid → HCID 178434171, compound

Participants

Reaction Conditions

Reaction Steps

To a 20 mL scintillation vial was charged ethyl 1-methyl-4-((3-methyl-6-(6-phenylpyridin-3-yl)-1H-dipyrazolo[1,5-a:4′,3′-e]pyrimidin-4-yl)methyl)cyclohexanecarboxylate (Int-6c, 0.03 mmol). To this residue was added 2:1 THF:H2O (1.5 mL) and LiOH.H2O (20 mg). The resulting solution was stirred at 80° C. for 4 days. After 4 days, the reaction was brought to pH 3 with 1N HCl(aq). The reaction was reduced in vacuo and the residue was purified via reverse-phase preparatory HPLC to 1-methyl-4-((3-methyl-6-(6-phenylpyridin-3-yl)-1H-dipyrazolo[1,5-a:4′,3′-e]pyrimidin-4-yl)methyl)cyclohexanecarboxylic acid (3). (M+H=481.27, retention time=4.01 min).

Provenance & Evidence