Lithium Hydroxide → HCID 135952366, compound

Participants

Reaction Steps

24 mg (0.04 mmol) of the compound from Example 8A were initially charged in 2.3 ml of a 2:1 mixture of dioxane and water, and 1.9 mg (0.08 mmol) of lithium hydroxide were added. The reaction mixture was stirred at RT for 4 h. A further 1.9 mg (0.08 mmol) of lithium hydroxide were added, and the mixture was stirred for another 4 h. 2 ml of water were added to the reaction. The mixture was extracted three times in total with in each case 4 ml of ethyl acetate. The combined organic phases were dried over magnesium sulfate and the solvent was removed on a rotary evaporator. The residue was purified by preparative HPLC (column: YMC GEL ODS-AQ S-5/15 μm; mobile phase gradient: acetonitrile/water 10:90→95:5).

Provenance & Evidence