HuagongsheHuagongshe Chemistry Print Report · For research reference only
Pyridoxal 5'-phosphate → (R)-(+)-1-Boc-3-aminopyrrolidine
Participants
| Role | Name | HCID | Charge |
|---|---|---|---|
| Reactant | Pyridoxal 5'-phosphate | HCID 1051 | — |
| Reactant | Nadide (JAN/USAN/INN) | HCID 5893 | — |
| Reactant | Sodium Hydroxide | HCID 14798 | — |
| Reactant | D-Alanine | HCID 71080 | — |
| Reactant | (2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanal | HCID 107526 | — |
| Reactant | Tert-butyl 3-oxopyrrolidine-1-carboxylate | HCID 471360 | — |
| Product | (R)-(+)-1-Boc-3-aminopyrrolidine | HCID 854070 | — |
Reaction Steps
To a flask containing 0.5 g of a substrate N-Boc-3-pyrrolidinone, 0.73 g of D-glucose, 4 mg of NAD+, 1.44 g of D-alanine, and 3.3 mg of pyridoxal phosphate, a culture fluid of the recombinant E. coli HB101 (pNTASPAG), obtained in Example 11, which expresses TAS, PALDH, and GDH was added so that total volume was 25 ml. The resultant product was stirred at 30° C. while being adjusted to a pH of 6.8 by drippage of 5 N aqueous solution of sodium hydroxide. After nine hours in reaction, 0.25 g of N-Boc-3-pyrrolidinone, 0.37 g of D-glucose, 0.37 g of D-alanine, and 3 mg of pyridoxal phosphate were added.
Provenance & Evidence
| DOI | 10.6084/m9.figshare.5104873.v1 |
|---|---|
| Patent number | US08168412B2 |
| Source dataset | uspto-grants-2012_05 |
| ORD ID | ord-fc3e45df8de8430f9df416e5bd68e269 |
| Source note | uspto-grants-2012_05 |