反应详情

To a solution of (3S,5S)-benzyl 3-(benzoyloxy)-5-(tert-butoxycarbonylamino)-piperidine-1-carboxylate (1 eq) in 15 methanol and 15 mL of EtOAc was added 10% Pd/C (0.1 eq). The resulting suspension was stirred at H2 atmosphere for 4 hours. The crude solids were filtered through a pad of Celite on a paper lined Buchner funnel, washed with MeOH, then concentrated in vacuo. The residue was dissolved in 20 mL of isopropanol and DIPEA (1.8 eq) and 4-chloro-3-nitropyridine (1.2 eq) were added. The reaction mixture was stirred at 75° C. for 2 hours, at which point the reaction mixture was allowed to cool to room temperature and concentrated under reduced pressure. The residue was diluted with 150 mL of EtOAc, washed with brine, then dried over anhydrous MgSO4, filtered, and concentrated in vacuo. The crude residue was purified by flash chromatography (EtOAc:hexanes=1:1) to yield (3S,5S)-5-(tert-butoxycarbonylamino)-1-(3-nitropyridin-4-yl)-piperidin-3-yl benzoate (90%). LC/MS (m/z): 443.2 (MH+). HPLC: Rt: 2.89 min.

1. filtrationThe crude solids were filtered through a pad of Celite on a paper
2. washwashed with MeOH
3. concentrationconcentrated in vacuo
4. dissolutionThe residue was dissolved in 20 mL of isopropanol
5. stirringThe reaction mixture was stirred at 75° C. for 2 hours, at which
6. concentrationconcentrated under reduced pressure
7. additionThe residue was diluted with 150 mL of EtOAc
8. washwashed with brine
9. dry with materialdried over anhydrous MgSO4
10. filtrationfiltered
11. concentrationconcentrated in vacuo
12. customThe crude residue was purified by flash chromatography (EtOAc:hexanes=1:1)