HuagongsheHuagongshe Chemistry Print Report · For research reference only
Sulfuric Acid → L-Glutamine
Participants
| Role | Name | HCID | Charge |
|---|---|---|---|
| Reactant | Sulfuric Acid | HCID 1118 | — |
| Reactant | Urea | HCID 1176 | — |
| Reactant | Calcium Carbonate | HCID 10112 | — |
| Reactant | Potassium Phosphate, Dibasic | HCID 24450 | — |
| Reactant | Magnesium Sulfate Heptahydrate | HCID 24843 | — |
| Reactant | (2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanal | HCID 107526 | — |
| Reactant | Biotin | HCID 171548 | — |
| Reactant | Monobasic potassium phosphate | HCID 516951 | — |
| Reactant | Ammonium Sulfate | HCID 6097028 | — |
| Reactant | Thiaminhydrochloride | HCID 67590811 | — |
| Catalyst | Ferrous sulfate heptahydrate | HCID 62662 | — |
| Catalyst | Manganese II sulfate pentahydrate | HCID 22138077 | — |
| Solvent | Water | HCID 962 | — |
| Product | L-Glutamine | HCID 5961 | — |
Reaction Steps
3 ml of each of the seed culture broths was inoculated to a 250-ml baffled conical flask containing 30 ml of a main culture medium [a medium comprising 50 g of glucose, 20 g of ammonium sulfate, 0.5 g of potassium dihydrogen phosphate, 0.5 g of dipotassium hydrogen phosphate, 2 g of urea, 0.5 g of magnesium sulfate heptahydrate, 2 mg of iron sulfate heptahydrate, 2.5 mg of manganese sulfate pentahydrate, 1 mg of thiamin hydrochloride, 100 μg of biotin, and 30 g of calcium carbonate, contained in 1 L of water, sterilized and then adjusted to pH 7.0 with sulfuric acid], and cultured under the conditions of 30° C. and 220 rpm for 16 hours.
Provenance & Evidence
| DOI | 10.6084/m9.figshare.5104873.v1 |
|---|---|
| Patent number | US08187843B2 |
| Source dataset | uspto-grants-2012_05 |
| ORD ID | ord-cb4e98edd47f462aabf9c452dbf45b2f |
| Source note | uspto-grants-2012_05 |