反应详情

温度

160 °C

A mixture of 4-(4-chloro-pyrazolo[3,4-d]pyrimidin-1-yl)-piperidine-1-carboxylic acid tert-butyl ester (Intermediate 19; 50 mg, 0.15 mmol), 6-methanesulfonyl-2-methyl-pyridin-3-ol (Intermediate 6; 36 mg, 0.19 mmol), and potassium carbonate (27 mg, 0.2 mmol) in dimethylformamide (2 mL) was heated in the microwave oven at 160° C. for 10 min. Ethyl acetate and water were added, and the aqueous layer was extracted three times with ethyl acetate. The ethyl acetate layers were combined, evaporated, and purified by preparative C18 HPLC, eluting with 10-100% acetonitrile/water. Samples containing the product were extracted with dichloromethane and the solvent was evaporated to give 4-[4-(6-methanesulfonyl-2-methyl-pyridin-3-yloxy)-pyrazolo[3,4-d]pyrimidin-1-yl]-piperidine-1-carboxylic acid tert-butyl ester (30 mg, 42%) as a white powder. 1H NMR (300 MHz, CDCl3) δ 1.50 (s, 9H), 1.96-2.06 (m, 2H), 2.20-2.34 (m, 2H), 2.92-3.05 (m, 2H), 4.26-4.39 (m, 2H), 4.92-5.04 (m, 1H), 7.76 (d, 1H, J=8.4 Hz), 8.06 (d, 1H, J=8.4 Hz), 8.21 (s, 1H), 8.46 (s, 1H). Mass spectrum (ES) MH+=489. HRMS Calcd. for C22H29N6O5S (MH+): 489.1915. Found: 489.1915.

1. extractionthe aqueous layer was extracted three times with ethyl acetate
2. customevaporated
3. custompurified by preparative C18 HPLC
4. washeluting with 10-100% acetonitrile/water
5. additionSamples containing the product
6. extractionwere extracted with dichloromethane
7. customthe solvent was evaporated