Ethyl Acetate → HCID 57965027, compound

Participants

Reaction Conditions

Reaction Steps

A mixture of 4-(4-chloro-pyrazolo[3,4-d]pyrimidin-1-yl)-piperidine-1-carboxylic acid tert-butyl ester (Intermediate 19; 50 mg, 0.15 mmol), 3-hydroxy-1-methylpyridazin-6(1H)-one (Oakwood Products, Inc., West Columbia, S.C., USA; 24 mg, 0.19 mmol), and potassium carbonate (27 mg, 0.2 mmol) in dimethylformamide (2 mL) was heated in the microwave oven at 160° C. for 10 min. Ethyl acetate and water were added, and the aqueous layer was extracted three times with ethyl acetate. The ethyl acetate layers were combined, evaporated, and purified by preparative C18 HPLC, eluting with 10-100% acetonitrile/water. Samples containing the product were extracted twice with dichloromethane and the solvent was evaporated under high vacuum to give 4-[4-(1-methyl-6-oxo-1,6-dihydro-pyridazin-3-yloxy)-pyrazolo[3,4-d]pyrimidin-1-yl]-piperidine-1-carboxylic acid tert-butyl ester (25 mg, 39%) as a white powder. 1H NMR (300 MHz, CDCl3) δ 1.49 (s, 9H), 1.95-2.04 (m, 2H), 2.20-2.36 (m, 2H), 2.92-3.05 (m, 2H), 3.76 (s, 3H), 4.26-4.40 (m, 2H), 4.90-5.02 (m, 1H), 7.07 (d, 1H, J=9.7 Hz), 7.24 (d, 1H, J=9.7 Hz), 8.17 (s, 1H), 8.51 (s, 1H). Mass spectrum (ES) MH+=428. HRMS Calcd. for C20H26N7O4 (MH+): 428.2041. Found: 428.2040.

Provenance & Evidence