HuagongsheHuagongshe Chemie-Druckbericht · Nur für Forschungszwecke
Phenylmethylsulfonyl Fluoride → L-Tyrosine
Beteiligte Stoffe
| Rolle | Name | HCID | Einwaage |
|---|---|---|---|
| Edukt | Phenylmethylsulfonyl Fluoride | HCID 4784 | — |
| Edukt | Sodium Fluoride | HCID 5235 | — |
| Edukt | Tris(hydroxymethyl)aminomethane | HCID 6503 | — |
| Edukt | Poly(oxy-1,2-ethanediyl), alpha-((1,1,3,3-tetramethylbutyl)phenyl)-omega-hydroxy-, potassium salt (1:1) | HCID 24775 | — |
| Edukt | Leupeptin acid | HCID 189069 | — |
| Lösungsmittel | Glycerol | HCID 753 | — |
| Produkt | L-Tyrosine | HCID 6057 | — |
Reaktionsschritte
Sf21 cells were infected with a baculovirus expression vector for BTK or SYK, as previously described (refs 18 and 19). Cells were harvested, lysed (10 mM Tris pH 7.6, 100 mM NaCl, 1% Nonidet P-40, 10% glycerol, 50 mM NaF, 100 mM Na3VO4, 50 μg/ml phenylmethylsulfonyl fluoride, 10 μg/ml aprotonin, 10 μg/ml leupeptin), and the kinase proteins were purified to homogeneity, as reported (refs 18 and 19).
Herkunft & Nachweise
| DOI | 10.6084/m9.figshare.5104873.v1 |
|---|---|
| Patentnummer | US06391921B1 |
| Quelldatensatz | uspto-grants-2002_05 |
| ORD ID | ord-6c1b4b0af82847228277616836d4b479 |
| Quellennotiz | uspto-grants-2002_05 |