Mercapto-ethanol → (2-Hydroxyethyldithio)ethylamidosuccinic acid

HRID 1940872Notes

Reaction equation

HRID 1940872 reaction equation
CO[C:3]([S:5][S:6][CH2:7][C:8]([NH:10][CH:11]([CH2:15][C:16]([OH:18])=[O:17])[C:12]([OH:14])=[O:13])=[O:9])=O.S[CH:20]([OH:22])C>CO>[OH:22][CH2:20][CH2:3][S:5][S:6][CH2:7][C:8]([NH:10][CH:11]([CH2:15][C:16]([OH:18])=[O:17])[C:12]([OH:14])=[O:13])=[O:9]

Participants

4

Reactant 2

HCID 4713212Mercapto-ethanolHCID 53797889(Methoxycarbonyldithio)ethylamidosuccinic acid

Product 1

HCID 54090062(2-Hydroxyethyldithio)ethylamidosuccinic acidYield 74.6%

Reagents, catalysts & solvents 1

HCID 887MethanolSolvent2×

Conditions

Temperature
0 °C

Procedure

A round bottomed flask was charged with 399.6 mg (1 eq., 1.495 mmole) (methoxycarbonyldithio)ethylamidosuccinic acid dissolved in 5 ml methanol, cooled to 0° C., purged with argon, and then treated dropwise with a solution of 110.9 mg (0.95 eq., 995.9 μl, 1.42 mmole) mercaptoethanol in 4 ml methanol over a 30 min period. The stirred solution was allowed to warm to room temperature over 1 hour and then stirred at this temperature for an additional 3.5 hours, after which, the solvent was evaporated on the rotary evaporator (bath temp. 30° C.). The residue was subjected to vacuum transfer for 0.5 hours to ensure that all mercaptoethanol was removed. The resultant white solid was then titurated with 3 ml ether and then the solid was pumped on the high vacuum for 10 min. (bath temp. 40° C.) to yield approximately 0.3 g of (2-hydroxyethyldithio)ethylamidosuccinic acid as a pure white solid. 1H NMR (d6DMSO/TMS) δ: 2.31 (2H, t, J=6.02 Hz, CH2), 2.41 (2H, t, J=6.39 Hz, CH2), 2.77 (4H, q, J=6.86 Hz, 2×CH2), 3.35 (2H, t, J=6.75 Hz, CH2), 3.60 (2H, t, J=6.46 Hz, CH2), 8.06 (1H, t, NH). 13C NMR (d6DMSO/TMS) δ: 30.53 (CH2), 31.62 (CH2), 38.46 (CH2), 39.68 (CH2), 42.11 (CH2), 61.27 (CH2), 174.76 (Q), 176.63 (Q).

Workup

  1. custompurged with argon
  2. temperatureto warm to room temperature over 1 hour
  3. customafter which, the solvent was evaporated on the rotary evaporator (bath temp. 30° C.)
  4. waitThe residue was subjected to vacuum transfer for 0.5 hours
  5. customwas removed
  6. waitthe solid was pumped on the high vacuum for 10 min.

Sources & evidence

ORD record
ord-cefdd9f7bc624432918498b1ee9d47d2
Source dataset
uspto-grants-1997_12
Patent number
US05700921

Reaction notes

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