Tromethamine hydrochloride → HCID 16680003, compound

Participants

Reaction Steps

L-Fucose (6-deoxy L-galactose) was reduced, for a microbial oxidation of the resulting L-fucitol (6-deoxy L-galactitol) with E. agglomerans 221e [the bacterial cell at OD600=30, Tris-HCl buffer (pH 8.0) of 50 mM, the substrate 6-deoxy L-galactitol at 1%] at 30° C. for 6 hours. Only 1-deoxy D-tagatose was produced. FIG. 44(a) shows the reaction mixture after reaction, while FIG. 44 (b) shows the results of HPLC analysis of the produced 1-deoxy D-tagatose. The optical rotation was +14.0. 13C NMR spectrum thereof is shown in FIG. 45.

Provenance & Evidence