Ampicillin → L-Lysine

HRID 1941751Notes

Reaction equation

HRID 1941751 reaction equation
CC1(C)S[C@@H]2[C@H](NC([C@H](N)C3C=CC=CC=3)=O)C(=O)[N:4]2[C@H]1C(O)=O.C[C@@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)[C@H](NC(N)=N)[C@@H](O)[C@@H]2NC(N)=N)[C@H]([O:49][C@@H:50]2[O:55][C@@H:54]([CH2:56]O)[C@H:53](O)[C@@H:52](O)[C@@H:51]2[NH:60]C)[C@@]1(O)C=O.C([O-])(=O)CCCCCCC/C=C\CCCCCCCC.[Na+]>OCC(CO)O>[NH2:60][C@H:51]([C:50]([OH:55])=[O:49])[CH2:52][CH2:53][CH2:54][CH2:56][NH2:4]

Participants

5

Reactant 3

HCID 6249Ampicillin2×HCID 23665730Sodium Oleate2×HCID 178484872HCID 178484872, compound2×

Product 1

HCID 5962L-Lysine

Reagents, catalysts & solvents 1

HCID 753GlycerolSolvent

Procedure

Glycerol stocks of the strains prepared in of Example 7 were thawed. 100 μL of each was evenly spread on an LB agar medium plate containing 40 mg/L ampicillin and 25 mg/L streptomycin and incubated at 37° C. for 24 hours. About ¼ volume of bacterial cells on the plate were inoculated into 40 mL of fermentation medium described below containing 40 mg/L ampicillin and 25 mg/L streptomycin in a 500 mL-Erlenmeyer flask equipped with baffles, and cultured using a reciprocal shaking culture apparatus at 37° C. for 48 hours. As a carbon source in a main culture, sodium oleate added with polyoxyethylene sorbitan monooleic acid ester (Tween 80: manufactured by NACALAI TESQUE, INC.) as an emulsification promoter to a final concentration of 0.5% (w/v) was used. As for a total amount of carbon sources, sodium oleate was 10 g/L. The medium composition used for the culture is shown below.

Workup

  1. customequipped with baffles
  2. customat 37° C.
  3. customfor 48 hours

Sources & evidence

ORD record
ord-3dcf4fa8cc064b04bd70839642cf970b
Source dataset
uspto-grants-2013_03
Patent number
US08389249B2

Reaction notes

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