Benzoylecgonine → HCID 131730269, compound

Participants

Reaction Steps

Benzoylecgonine (BE) (4 mg) was dissolved in dry dioxin (500 ul) and N-hydroxysuccinamide (NHS) (4 mg) added. 1-Ethyl-3-(3-dimethylaminopropul)carbodiimide hydrochloride (8 mg) was suspended/dissolved in dioxan (1 ml) and immediately added to the mixture. The mixture was shaken at ambient temperature for 2 hours (to form BE-succinamide). To this was added diamino terminated polyethyleneglycol (mol. wt. 20,000) dissolved in 1 ml of 0.1M sodium bicarbonate solution. The solution was left overnight (about 16 hrs) and was then diluted with tris/triton buffer.

Provenance & Evidence