5'-Atp → 5'-Adp

Participants

Reaction Steps

Next, 50 μl of a reaction mixture containing 0.16 μg of the apyrase-treated PPK-ADK, 10 μM AMP, 400 μM polyphosphate, 8 mM MgCl2, and 60 mM Tris-HCl (pH 7.4) were prepared. Then, 5 μl of the reaction mixture were sampled and mixed with 40 μl of an ATP bioluminescence assay reagent (Roche), and luminescence was measured immediately by using a multiplate luminometer (ARVO, Wallac).

Provenance & Evidence