Sodium Hydroxide → HCID 67435012, compound

Participants

Reaction Steps

Intermediate 35 (496 mg) was dissolved in ethanol (5 ml) and the resulting soln. was treated with sodium hydroxide, 2M aq (3 ml). The resulting suspension was heated to reflux for 4 hr. The reaction mixture was allowed to cool and concentrated in vacuo. The crude residue was taken up in acetic acid (10.00 ml) and heated to reflux for 1 hr. Reaction mixture concentrated in vacuo. The residue was purified by reverse phase chromatography using acetonitrile water with an ammonium carbonate modifier gradient. The desired fractions were combined and concentrated under vacuum to give the title compound (304 mg) as a white solid. LC/MS MH+ 347, Rt 1.93 min (5 minute run).

Provenance & Evidence