Water → HCID 71205727, compound

Participants

Reaction Steps

Intermediate 51 (170 mg) was dissolved in 2 ml methanol followed by 4 M equivalent of sodium hydroxide and the mixture stirred at room temperature for 24 hr. The reaction mixture was reduced in vacuo and dissolved in a minimum amount of 1:1 acetonitrile:water. This mixture was passed through an SCX ion exchange column (5 g). The column was washed with 1:1 acetonitrile:water, followed by acetonitrile. The product was eluted with 2M ammonia in methanol and reduced in vacuo. LC/MS MH+ 372, Rt 2.12 mins (5 min run, TFA modifier).

Provenance & Evidence