Hydrochloric Acid → HCID 67434993, compound

Participants

Reaction Steps

Intermediate 66 (177 mg) was dissolved in methanol (4 mL). NaOH (2 mL, 6.60 mmol) was added to the stirred reaction mixture as a 3.3 N aqueous soln and the reaction mixture. After stirring overnight the reaction mixture was conc in vacuo and taken up in acetonitrile/water. The soln was taken to pH4-5 by the addition of 2 M aqueous HCl and loaded onto an SCX column. After washing with water and acetonitrile, the product was eluted with 2 M ammonia soln in methanol. The washing were conc in vacuo to afford the ammonium salt of the desired product. LC/MS, MH+ 361, Rt 2.01 mins (5 min system).

Provenance & Evidence